Issue |
Eur. Phys. J. Appl. Phys.
Volume 91, Number 3, September 2020
|
|
---|---|---|
Article Number | 30701 | |
Number of page(s) | 8 | |
Section | Imaging, Microscopy and Spectroscopy | |
DOI | https://doi.org/10.1051/epjap/2020200101 | |
Published online | 18 September 2020 |
https://doi.org/10.1051/epjap/2020200101
Regular Article
Contrast-tuneable microscopy for single-shot real-time imaging
1
Research Institute of Advanced Technology, Inc. 3543-2 Higashi-ishikawa, Hitachinaka-Shi, Ibaraki 312-0052, Japan
2
Department of Media and Telecommunications Engineering, College of Engineering, Ibaraki University, 4-12-1 Naka-Narusawa, Hitachi, Ibaraki 316-8511, Japan
3
Department of Applied Physics, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573 Japan
4
York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5DD, United Kingdom
* e-mail: riat-smz@riat.co.jp, seishi.shimizu@york.ac.uk
Received:
12
April
2020
Received in final form:
23
July
2020
Accepted:
12
August
2020
Published online: 18 September 2020
A novel real image in-line laser holography has enabled a tuneable image contrast, edge sharpness, and visualization of sub-wavelength structures, using a simple pair of filters and large-diameter lenses that can incorporate higher-order scattered beams. Demonstrated also are the accuracy in object sizing and the ease of imaging along the focal depth, based on a single-shot imaging via holographic principle. In addition, the use of broad, collimated laser beam for irradiation has led to a wider field of view, making it particularly useful for an extensive monitoring of, and sweeping search for, cells and microbial colonies and for the real-time imaging of cancer-cell dynamics.
© EDP Sciences, 2020
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