| Issue |
Eur. Phys. J. AP
Volume 19, Number 1, July 2002
|
|
|---|---|---|
| Page(s) | 55 - 62 | |
| Section | Physics of Energy Generation, Conversion and Storage | |
| DOI | https://doi.org/10.1051/epjap:2002048 | |
| Published online | 28 June 2002 | |
https://doi.org/10.1051/epjap:2002048
Spectrophotometry for the characterisation of yeast cells suspensions
1
Università degli Studi di Milano-Bicocca, Dipartimento di Fisica “G. Occhialini”,
Piazza della Scienza 3 and Istituto Nazionale di Fisica della
Materia, unità di Milano-Bicocca, 20126 Milano, Italy
2
Dipartimento di Elettronica e
Informazione, Politecnico di Milano, P.za Leonardo da Vinci and Istituto Nazionale di
Fisica della Materia, unità di Milano-Bicocca, 20133 Milano, Italy
3
Università degli Studi
di Milano - Bicocca, Dipartimento di Scienze dei Materiali, Via Cozzi and Istituto
Nazionale di Fisica della Materia, unità di Milano-Bicocca, 20126 Milano, Italy
Corresponding author: batani@mib.infn.it
Received:
18
July
2001
Accepted:
28
March
2002
Published online:
28
June
2002
The use of spectrophotometers for the measurement of cell concentration in yeast suspension is critically discussed. Corrections are derived which take into account cell size, shape, cell aggregation (flocculation) and non linear effects related to sample thickness.
PACS: 87.80.-y – Biological techniques and instrumentation; biomedical engineering / 87.64.-t – Spectroscopic and microscopic techniques in biophysics and medical physics
© EDP Sciences, 2002
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