Eur. Phys. J. AP
Volume 17, Number 2, February 2002
|Page(s)||155 - 162|
|Section||Physics of Energy Generation, Conversion and Storage|
|Published online||15 February 2002|
Effect of shear stress and of transmural pressure on cAMP-dependent responses of cells adhering to a biomaterial
Biomécanique et Génie Biomédical (UMR CNRS 6600) ,
Université de Technologie de Compiègne, BP 20529, 60205 Compiègne Cedex, France
Revised: 30 October 2001
Accepted: 15 November 2001
Published online: 15 February 2002
Biomaterials used in some bioreactors are porous and exposed to normal and tangential flow of physiological fluid. Flow-induced forces may influence the morphological and biochemical responses of cells adhering to these materials. The objective of this work is to examine the capacity of mechanical stress to cause changes in cell morphology via the cAMP pathway (cyclic adenosine monophosphate). This second messenger is known to modulate cell morphology in static conditions. In classical flow devices, cells are submitted to only tangential stresses. We designed a new flow system, a Hele-Shaw cell with a porous bottom wall, in order to take into account the influence of a transmural pressure. This flow chamber allows to follow up continuously the shape changes of cells that are adherent to a porous biomaterial (polyacrylonitrile) and are exposed to controlled levels of shear stress or transmural pressure. Mouse Swiss 3T3 fibroblasts exposed to a 1.1-Pa shear stress, as well as those exposed to a 84-mm Hg transmural pressure, round up (up to 50%) in a few minutes. If the cAMP pathway is inhibited when a mechanical stress is applied, cell rounding is significantly prevented. These observations suggest that flow-induced cell shape changes are cAMP-dependent. This conclusion is supported by an increased cAMP accumulation measured in cells under mechanical stress when compared to static experiments. Our in vitro flow system is thus useful to study the influence of transmural pressure or shear stress on the early morphological and biochemical responses of cells in contact with a biomaterial.
PACS: 47.15.Gf – Low-Reynolds-number (creeping) flows / 87.16.Xa – Signal transduction / 87.68.+z – Biomaterials and biological interfaces
© EDP Sciences, 2002
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.