Surface-enhanced Raman spectra of hemoglobin for esophageal cancer diagnosis

¹ College of Physical Science and Engineering, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
² Application Institute of Spectroscopy Technology, Chuxiong Normal University, Chuxiong, Yunan 675000, P.R. China

^a e-mail: 185226112@qq.com

Received: 6 September 2013
Revised: 6 December 2013
Accepted: 16 January 2014
Published online: 3 March 2014

Abstract

Surface-enhanced Raman scattering (SERS) spectra of hemoglobin from 30 esophageal cancer patients and 30 healthy persons have been detected and analyzed. The results indicate that, there are more iron ions in low spin state and less in high for the hemoglobin of esophageal cancer patients than normal persons, which is consistent with the fact that it is easier to hemolyze for the blood of cancer patients. By using principal component analysis (PCA) and discriminate analysis, we can get a three-dimensional scatter plot of PC scores from the SERS spectra of healthy persons and cancer patients, from which the two groups can be discriminated. The total accuracy of this method is 90%, while the diagnostic specificity is 93.3% and sensitivity is 86.7%. Thus SERS spectra of hemoglobin analysis combined with PCA may be a new technique for the early diagnose of esophageal cancer.