Eur. Phys. J. Appl. Phys.
Volume 56, Number 1, October 2011
Topical Issue: 5th Colloquium Interdisciplinary in Instrumentation (C2I) 2010
|Number of page(s)||5|
|Published online||27 September 2011|
Antigen-antibody selective recognition using LiTaO3 SH-SAW sensors: investigations on macromolecules effects on binding kinetic constants
Unité de Recherche de Physique des Semiconducteurs et Capteurs, IPEST, 2070 La Marsa, Tunisia
2 Laboratoire de Physique, EA 4131, Cnam, 2 rue Conté, 75003 Paris, France
Accepted: 10 January 2011
Published online: 28 September 2011
A gravimetric surface acoustic wave (SAW) biosensor, based on the biotin-streptavidin and antistreptavidin-streptavidin recognitions, has been carried out. A network analyser and a pulse excitation technique were used to monitor both amplitude and phase changes. The SAW biosensor presented a total selective recognition of streptavidin-antistreptavidin and HRPstreptavidin-antistreptavidin. The presence of HRP (Horseradish peroxidase) affects neither the selectivity nor the sensitivity (of order of 0.25°/nM) of the biosensor, nevertheless, it causes a reduction of binding kinetics by a factor ranging between 2 to 5, as well as a diminution of antistreptavidin saturation concentration (of 40%). Results showed that equilibrium constants can be different, depending on evaluation method (from saturation values or from linear part of the output signal variation according to solution concentration).
© EDP Sciences, 2011
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.