| Issue |
Eur. Phys. J. Appl. Phys.
Volume 44, Number 1, October 2008
10th Meeting of the French Microscopy Society (SFMU)
|
|
|---|---|---|
| Page(s) | 29 - 35 | |
| Section | Instrumentation and Metrology | |
| DOI | https://doi.org/10.1051/epjap:2008049 | |
| Published online | 16 April 2008 | |
https://doi.org/10.1051/epjap:2008049
Tomographic diffractive microscopy of transparent samples
1
Laboratoire MIPS-LabEl, IUT Mulhouse, 61 rue A. Camus 68093 Mulhouse Cedex, France
2
Lauer Microscopie, 1bis rue des Blés, 68200 Mulhouse, France
Corresponding author: olivier.haeberle@uha.fr
Received:
13
September
2007
Accepted:
17
October
2007
Published online:
16
April
2008
We report a tomographic diffractive microscope, which permits imaging non-labelled transparent or semi-transparent samples. Based on a combination of microholography with a tomographic illumination, our set-up creates 3-D images of the index of refraction distribution within the sample. One acquires successively interferograms, rotating the illumination (the specimen being static) and using phase-shifting holography. Within the first Born approximation, each interferogram is interpreted as a subset of the Fourier transform of the specimen index of refraction distribution. The reconstruction is therefore similar to synthetic aperture imaging: one recombines the information in the Fourier space, and a final Fourier transform gives a 3-D image of the specimen. First recalling the theoretical foundations, we then describe our experiment, and show initial results obtained on biological samples.
PACS: 42.30.Wb – Image reconstruction; tomography / 42.30.Kq – Fourier optics / 42.40.Kw – Holographic interferometry; other holographic techniques
© EDP Sciences, 2008
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