Eur. Phys. J. Appl. Phys.
Volume 44, Number 1, October 2008
10th Meeting of the French Microscopy Society (SFMU)
|Page(s)||29 - 35|
|Section||Instrumentation and Metrology|
|Published online||16 April 2008|
Tomographic diffractive microscopy of transparent samples
Laboratoire MIPS-LabEl, IUT Mulhouse, 61 rue A. Camus 68093 Mulhouse Cedex, France
2 Lauer Microscopie, 1bis rue des Blés, 68200 Mulhouse, France
Corresponding author: firstname.lastname@example.org
Accepted: 17 October 2007
Published online: 16 April 2008
We report a tomographic diffractive microscope, which permits imaging non-labelled transparent or semi-transparent samples. Based on a combination of microholography with a tomographic illumination, our set-up creates 3-D images of the index of refraction distribution within the sample. One acquires successively interferograms, rotating the illumination (the specimen being static) and using phase-shifting holography. Within the first Born approximation, each interferogram is interpreted as a subset of the Fourier transform of the specimen index of refraction distribution. The reconstruction is therefore similar to synthetic aperture imaging: one recombines the information in the Fourier space, and a final Fourier transform gives a 3-D image of the specimen. First recalling the theoretical foundations, we then describe our experiment, and show initial results obtained on biological samples.
PACS: 42.30.Wb – Image reconstruction; tomography / 42.30.Kq – Fourier optics / 42.40.Kw – Holographic interferometry; other holographic techniques
© EDP Sciences, 2008
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