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Issue		Eur. Phys. J. Appl. Phys. Volume 5, Number 1, January 1999
Page(s)		101 - 109
DOI		10.1051/epjap:1999116

DOI: 10.1051/epjap:1999116

Eur. Phys. J. AP 5, 101-109

Yeast cell metabolism investigated by CO₂ production and soft X-ray irradiation

A. Masini^1,5 - D. Batani^1,5 - F. Previdi^2,5 - M. Milani^3,5 - A. Pozzi¹ - E. Turcu⁴ - S. Huntington⁴ - H. Takeyasu⁴

¹ Dipartimento di Fisica, Seconda Università di Milano Bicocca, via Emanueli 15, 20126 Milano, Italy
² Dipartimento di Elettronica e Informazione, Politecnico di Milano, via Ponzio 34/5, 20133 Milano, Italy
³ Dipartimento di Scienza dei Materiali, Seconda Università di Milano Bicocca, via Emanueli 15, 20126 Milano, Italy
⁴ Rutherford Appleton Laboratory, Chilton Didcot, Oxon, OX11 OQX, UK
⁵ INFM, Istituto Nazionale di Fisica della Materia, Milano, Italy
previdi@elet.polimi.it

Received: 17 June 1998 / Accepted: 24 September 1998

Abstract
Results obtained using a new technique for studying cell metabolism are presented. The technique, consisting in CO₂ production monitoring, has been applied to Saccharomyces cerevisiae yeast cells. Also the cells were irradiated using the soft X-ray laser-plasma source at Rutherford Appleton Laboratory with the aim of producing a damage of metabolic processes at the wall level, responsible for fermentation, without great interference with respiration, taking place in mitochondria, and DNA activity. The source was calibrated with PIN diodes and X-ray spectrometers and used Teflon stripes as target, emitting X-rays at about 0.9 keV, with a very low penetration in biological material. X-ray doses delivered to the different cell compartments were calculated following a Lambert-Bouguet-Beer law. Immediately after irradiation, the damage to metabolic activity was measured again by monitoring CO₂ production. Results showed a general reduction in gas production by irradiated samples, together with non-linear and non-monotone response to dose. There was also evidence of oscillations in cell metabolic activity and of X-ray induced changes in oscillation frequency.

PACS
87.22.-q Physics of cellular and physiological processes - 87.50.-a Biological effects of electromagnetic and particle radiation - 52.40.Nk Laser-plasma interaction

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