The European Physical Journal Applied Physics

Research Article

Characterization of microporous membranes using confocal scanning laser microscopy in fluorescence mode

C. Charcosseta1 and J.-C. Bernengoa2

Laboratoire d'Automatique et de Génie des Procédés, UCB Lyon 1, UPRES A-Q 5007 CNRS, ESCPE-Lyon, Bât. 308 G, 43 boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France

UCB Lyon 1, Centre Commun de Quantimétrie, 8 avenue Rockfeller, 69373 Lyon Cedex 08, France

Abstract

Confocal Scanning Laser Microscopy (CSLM) in fluorescence mode was used to characterize microporous membranes. Two microfiltration membranes were investigated: a mixed ester (cellulose nitrate/cellulose acetate) 1.2 μm-rated membrane and a polycarbonate track-etched membrane with cylindrical pores of 2 μm diameter. Optical sections of the membranes stained with rhodamine and mounted in glycerol were performed at 1 μm intervals, from 0 to 10 μm. CSLM was found useful for microporous membrane characterization, as it gives some insight into bulk membrane morphology.

(Received May 16 2000)

(Revised July 12 2000)

(Accepted September 15 2000)

(Online publication December 15 2000)

PACS:

  • 81.05.Rm – Porous materials; granular materials;
  • 87.64.Tt – Confocal microscopy;
  • 81.70.Fy – Nondestructive testing: optical methods