ESPCI, LPEM de la Ville de Paris, CNRS UPR5, Université Pierre et Marie Curie, Paris 6, 10, rue Vauquelin, 75231 Paris Cedex 05, France
ENS, LKB, CNRS UMR 8552, Université Pierre et Marie Curie, Paris 6, 24, rue Lhomond, 75231 Paris Cedex 05, France
Most applications involving metallic nanoparticle labelling necessitate both fast imaging and local spectroscopy of individual beads. We present an imaging method based on a guided laser illumination associated to spatial modulation which allows the detection of gold particles down to 10 nm. Using an imaging spectrometer and white incoherent illumination, the same system delivers individual spectroscopy of several gold beads simultaneously, allowing a fast and selective discrimination between individual metal particles and aggregates or dust.
(Received January 29 2008)
(Revised April 3 2008)
(Accepted April 30 2008)
(Online publication July 10 2008)