Genetical Research

Genetic analysis of an intersex allele (ix5) that regulates sexual phenotype of both female and male Drosophila melanogaster

M.  ACHARYYA  a1 and R. N.  CHATTERJEE  a1 c1
a1 Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700 019, India


An allele of intersex (ix5) of Drosophila melanogaster has been characterized. The genetic analysis of the allele demonstrated that like other point mutations of ix, the ix5 allele also transformed diplo-X individuals into intersexes. The ix5 mutation also affects the arrangement of sex comb bristles on the forelegs of males, although they had morphologically nearly normal male genitalia. They often fail to display a sustained pattern of courtship activity when tested. Orcein-stained squash preparations of testes from ix5 males revealed a defect in spermatogenesis. Our results, taken together with those of McRobert & Tompkins (1985), indicate that the ix+ gene also functions in male sex determination.

(Received September 5 2001)
(Revised January 21 2002)

c1 Corresponding author. e-mail: